Bio-Rad ChromLab™ Software - Manual (Page 249)

Evaluating Fractions
User Guide | 247
 Tube location — the location of the group of pooled tubes in the rack.
 Start —the beginning of the collection of the first fraction in the pool.
 End — the end of the collection of the last fraction in the pool.
 Collected volume (ml) — the total fraction volume collected in all tubes in
the pool.
 Area (ml*mAU) — the sum of all areas in the pool. This column appears
after peak integration is performed.
 Relative area (%) — the relative pool area of all areas in a trace. This
column appears after peak integration is performed.
 Amount (mg) — the sum of all fraction amounts in the pool. This column
appears after peak integration is performed.
 Concentration (mg/ml) — the calculated protein concentration for the
specified pool. This value is the weighted average by volume of the fraction
concentrations in the pool.
 Molarity (
M) — the calculated molar concentration of the protein for the
pool. This value is the weighted average by volume of the molar
concentrations for the fractions in the pool.
To create a fraction pool
1. In the Fractions table, hold down Ctrl or Shift and select multiple adjacent rows
of fractions.
2. Right-click the group of adjacent fractions and select Pool.
To expand the pool in the Fractions table
 Do one of the following:
 In the Fractions table, click the plus sign in the first cell.
 Right-click the Fractions table and select Expand all pools.
To clear a pool
 In the Fractions table, right-click the pool row and select Unpool.