Bio-Rad ChromLab™ Software - Manual (Page 125)

Method Steps Pane
User Guide | 123
In this pane you can define the parameters of each method phase. Parameters vary
depending on the phase and the devices available on the system that is to
participate in that method phase. Some global method parameters defined in the
Method Settings phase can be altered for each phase. Such parameters include
flow rate, buffer selection, pH, and fraction volume.
Method Steps Pane
Visible when the Method Steps tab is selected, the Method Steps pane displays a
table of all steps associated with the method and their detailed settings. Use this
pane to modify phases and to create custom phases.
When a SIM is present in the fluidic scheme with an autosampler, SIM control
appears in the Step Description column when the autosampler is activated. When a
SIM is present in the fluidic scheme without an autosampler, SIM Control appears in
the Step Description column and the output name and pulse type for the device
appear in the Step Parameters column. The pulse type changes to from High to Low
when sample injection starts and returns to High when the injection stops.
When the method is a scout, the table includes a Scout column. The scouted steps
are identified with a check mark in the Scout column.