Bio-Rad ChromLab™ Software - Manual (Page 193)

Scouting on Parameters within a Method
User Guide | 191
Generating Column Scout Sequences
 This option is available only when the fluidic scheme includes the column
switching valve.
 If you chose Custom as the column type and changed the default values
for column volume, maximum precolumn pressure, and/or maximum delta-
column pressure, your custom settings must be within the system limits
defined by the method settings. If your custom values are outside of this
range, a red box appears around the number. The wizard prevents you
from continuing until you enter a valid number.
Generating Sample Scout Sequences
 This option is available only when the fluidic scheme includes at least one
sample inlet valve and you chose to load the loop through the sample
pump or inject sample directly onto the column in the Sample Application
phase. It is not available if you chose to load the loop manually.
 If your method contains multiple Sample Application phases, scouting is
performed only on the first sample loading step in the method.
 If you select Load Loop with Sample Pump in the method, the injection
volume in the scout refers to the system pump injection volume, the initial
injection volume is the same as the system pump injection volume set in
the method, and the minimum injection volume is 0.01 ml.
 If you select Inject Sample on Column with Sample Pump in the method,
the injection volume in the scout refers to the sample pump injection
volume, the initial injection volume is the same as the sample pump
injection volume set in the method, and the minimum injection volume is
0.01 ml.
 The maximum injection volume is 1,000 L.