Bio-Rad ChromLab™ Software - Manual (Page 293)

Performing Multicolumn Purification Chromatography
User Guide | 291
Performing Multicolumn Purification Chromatography
To perform multicolumn purification chromatography on an NGC system you must
 Plumb the NGC system
 Prime the NGC system
 Select and save a Method template
 Run the method
Plumbing the NGC System
How you set up and plumb the NGC system depends on the type of method you
plan to run. This section provides information about plumbing the system to use the
multicolumn purification templates.
Plumbing NGC Discover Pro Systems
This table lists the tasks for plumbing NGC Discover™ Pro systems. See Chapter 5,
Plumbing and Priming the NGC Chromatography Systems, in the NGC
Chromatography Software Installation Guide for detailed instructions.
Plumbing NGC Discover Pro Systems
Task
1. Tube #6 between the outlet port on the sample inlet valve and the
inlet port on the sample pump
2. Connect one of the following:
 Tube #4 from the Common inlet port on the BioFrac fraction
collector diverter valve to port 1 on the outlet valve
 Waste tubing to port 1 on the outlet valve
3. Inlet tubing to the remaining ports on the sample inlet valve
4. Outlet tubing to the remaining ports on the outlet valve
Note: Remove the inlet tubing from the sample pump inlet
manifold if it is installed.