Bio-Rad ChromLab™ Software - Manual (Page 292)

B | Multicolumn Purifications
290 | NGC Chromatography Systems and ChromLab Software
2-D Affinity (5 ml) > Desalting (50 ml) This method is used to purify up to four samples. Using a
sample inlet valve and the sample pump, the method
loads different samples onto separate affinity columns
and washes unbound material off in quick succession.
This process may be repeated for up to four columns. This
method is designed to reduce the target protein’s
exposure to proteases. The target proteins are eluted from
each column sequentially using a step gradient. The
eluted fraction is stored in a loop valve (a column
switching valve plumbed to the loop ports on the sample
inject valve with multiple sample loops). The fraction is
then injected onto a desalting column to perform a buffer
exchange or to remove salt.
Affinity (1 ml) > Desalting (10 ml) This method is used to purify up to five samples. Using a
sample inlet valve and the sample pump, the method
loads different samples onto separate affinity columns
and washes unbound material off in quick succession.
This process may be repeated for up to five columns. This
method is designed to reduce the target protein’s
exposure to proteases. The target proteins are eluted from
each column sequentially using a step gradient. Each
eluted fraction is then passed immediately through a
desalting column to perform a buffer exchange or to
remove salt.
Affinity (5 ml) > Desalting (50 ml) This method is used to purify up to five samples. Using a
sample inlet valve and the sample pump, the method
loads different samples onto separate affinity columns
and washes unbound material off in quick succession.
This process may be repeated for up to five columns. This
method is designed to reduce the target protein’s
exposure to proteases. The target proteins are eluted from
each column sequentially using a step gradient. Each
eluted fraction is then passed immediately through a
desalting column to perform a buffer exchange or to
remove salt.
Table 8. Multicolumn tandem templates, continued
Template Name Description