Bio-Rad ChromLab™ Software - Manual (Page 110)

4 | Performing a Manual Run
108 | NGC Chromatography Systems and ChromLab Software
To run an experiment step
1. Isocratic or gradient step:
a. In the System Pump module dialog box, under Flow Control, set the Flow
Rate, Mode (Isocratic or Gradient), %B value, Start and End values (in
Gradient mode), and Duration (optional for Isocratic mode) for the run.
b. Click Start to begin the run.
c. Minimize the Sample Inject Valve dialog box.
2. Sample injection step:
a. With the valve in the Manual Load Loop position, use a syringe to inject
sample through the sample inject port into the sample loop.
b. Change the valve position from Manual Load Loop to Inject Sample.
c. When the sample finishes injecting, switch the valve back to Manual Load
Loop.