Bio-Rad ChromLab™ Software - Manual (Page 245)

Evaluating Fractions
User Guide | 243
Displaying Fraction Collection Results — the
Fractions Table
Like the Traces table, the Fractions table is available when the run is opened in the
Evaluation window. The Fractions table is available only when a single run is open in
the Evaluation window. It is not displayed for multiple run analyses or for trace
comparisons.
The following fields are displayed for each fraction collected:
 # — sequential row number.
 Rack/Tube — the rack and tube number of the fraction.
 Tube location — the location of the tube in the plate or rack based on its
numbering scheme.
 Start — the beginning of the collection, displayed in the units of the
currently displayed x-axis.
 End — the end of the collection, displayed in the units of the currently
displayed x-axis.
 Collected volume (ml) — the total fraction volume collected in that tube.
 Area (ml*mAU) — the area of the fraction between the UV trace selected
in the heat map and its baseline. This column appears after peak
integration is performed.
 Amount (mg) — the amount of protein in the fraction based on the trace
selected in the heat map. This column appears after peak integration is
performed and the extinction coefficient value is entered into the table.
 Concentration (mg/ml) — the calculated concentration of the protein for
the specified fraction based on the trace selected in the heat map. This
column appears after peak integration is performed and the extinction
coefficient value is entered into the table.
 Relative area (%) — the relative fraction area of all fraction areas in a
trace. This column appears after peak integration is performed.