Adding phases, Editing methods and phases, Adding phases editing methods and phases – Bio-Rad ChromLab™ Software User Manual

Page 172

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6

| Creating a Method

170

| NGC Chromatography Systems and ChromLab Software

about priming your system.

9.

Select a pump head type. To set the control parameters see

Control Flow Tab

on page

72

.

10. Under Buffer Selection, select buffers for each inlet line or select the buffer

system and pH if you are using the buffer blending module. See also

Buffer

Selection on page

133

.

11. Click Rename Ports to change the names Buffer A and Buffer B to more

descriptive terms.

Names you select here appear in the method report.

Adding Phases

When you add a phase to a method, the parameters for the added phase appear in

the Method Outline pane.

To add phases to a method

1.

Drag a phase from the Phase Library pane to a position in the Method Outline
pane.

2.

Continue adding phases until the method is complete.

3.

Save the method.

Editing Methods and Phases

Changes you make to the Method Settings phase affect the entire method.

Changes you make to any other phase apply only to that phase.

To edit phases in the Phase Parameters pane

1.

With the Phase Parameters tab selected, select a phase in the method’s
Method Outline pane.

The settings for the selected phase appear in the Phase Parameters pane.

2.

Edit the phase parameter settings.

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