Viewing fraction collection results — the heat map – Bio-Rad ChromLab™ Software User Manual

Page 250

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7

| Evaluating Results

248

| NGC Chromatography Systems and ChromLab Software

Viewing Fraction Collection Results — the Heat Map

A configuration of the fraction collector (the rack display or the outlet valve) appears

to the left of the Fractions table. The image displays the configuration of the

selected fraction collector when the run was executed. After peak integration, a

heat map of the different fraction properties appears. If a multi-wavelength UV

detector was used for the run, you can select a trace for the heat map (the default

trace is 280 nm). The color of the heat map is based on the color of the chosen trace

in the chromatogram. Tubes or outlet valve ports that do not contain fractions

appear shaded.

Note:

Because OV1 Port 1 directs flow either to the diverter valve of the

BioFrac fraction collector or to waste, it always appears shaded in the outlet

valve display. When two outlet valves are used to collect fractions, OV1Port 12

also appears shaded.

You can base the heat map on any of the following columns in the Fractions table by

selecting from the Color by dropdown list:

Area

Concentration

Amount

Molarity

280/260 (or 255)

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