Peak integration – Bio-Rad ChromLab™ Software User Manual

Page 229

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Peak Integration

User Guide

| 227

Peak Integration

ChromLab software uses an algorithm to find peaks in UV traces and calculate the

necessary data for analysis, such as retention volume and peak area. The results

appear in the Peaks table at the bottom of the Evaluation window and in the

chromatogram.

You can perform peak integration on a single run or on multiple runs in a single view.

When you click Peak Integration on the Run tab toolbar, peak integration is

performed automatically using default settings, which appear in the Peak

Integration pane to the right of the chromatogram. You can change the parameter

settings or the traces selected for integration and run peak integration again with

the new settings.

You can also adjust peaks manually. Doing so adjusts only the selected trace and

displays only this trace’s peaks in the chromatogram. See

Adjusting Peaks Manually

on page

238

for details.

Tip:

SIM traces for external detectors connected to the NGC instruments are

also used for peak detection and integration. In this case, the y-axis units

defined in System Settings for the external detectors are used for peak

calculations. See

Device Input Tab on page

76

for more information.

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