Creating a method from standard phases – Bio-Rad ChromLab™ Software User Manual

Page 167

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Creating a Method from Standard Phases

User Guide

| 165

6.

Do one of the following:

Select a project folder. In the Method Name box, type a name for the

method, and then click Save.

Click New SubProject or New Root Project and type a name for the new

project in the Enter Project Name box that appears above the Projects

pane. Click Save.

Creating a Method from Standard Phases

When you create a method from standard phases, you specify in the Phase

Parameters pane the general settings that will apply to the entire method. These

settings include column selection, pressure limits, flow rate, method base, pH,

fraction collector rack type, and fraction volume. The parameters available in this

pane vary depending on the devices present in the fluidic scheme. For example, pH

is available only when the pH valve is present.

The workflow for creating a method from standard phases is as follows:

Creating a method.

Selecting a fluidic scheme.

Important:

The fluidic scheme must match the configuration of the NGC

instrument. If it does not, change the fluidic scheme or create a new one to

match the NGC instrument configuration. See

To change the fluidic

scheme on page

166

in this section.

Adding phases to the method by dragging them from the Phase Library

pane to the Method Outline pane in any position below the Method

Settings phase.

Editing the properties of the phases.

Saving the method.

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