B multicolumn purifications, Appendix b, Multicolumn purifications – Bio-Rad ChromLab™ Software User Manual
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B
Multicolumn Purifications
Protein purification can involve challenging separations of
complex mixtures that
might not provide sufficient resolution of target proteins from their impurities in a
single chromatographic step. Such cases may require multiple discrete experiments
involving multiple columns and different column chemistries.
Multicolumn purifications combine a series of columns, often with different or
orthogonal chemistries in sequence or in tandem, in a single separation protocol.
Most common protein purification techniques can be incorporated into a single
protocol utilizing multiple columns, providing convenience through automation for
proteins purified on a routine basis. A typical example of a multicolumn
chromatographic scheme consists of an affinity purification step, via a histidine (His)
or glutathione-S-transferase (GST) tag, followed by one or more additional
experiments involving size exclusion columns for desalting or aggregate removal, or
ion exchange columns for separating complex mixtures.
To streamline the combination of such disparate chromatographic methods, an
automated chromatography system with a flexible design
and versatile control
software is essential.
With their flexibility and scalability NGC™ chromatography
systems can be easily customized to meet your multicolumn purification
requirements.