Bio-Rad ChromLab™ Software - Manual (Page 290)

B | Multicolumn Purifications
288 | NGC Chromatography Systems and ChromLab Software
Affinity (5 ml) — Step Gradient This method is used to purify many samples quickly. Using a
sample inlet valve and the sample pump, the method loads
sample onto separate affinity columns and washes unbound
material off in quick succession. This process can be repeated
for up to five columns. This method is designed to reduce the
target protein’s exposure to proteases. The target proteins are
then eluted from each column sequentially using a step
gradient.
Table 7. Multicolumn sequential templates, continued
Template Name Template Description