Bio-Rad ChromLab™ Software User Manual

Page 39

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Chromatogram View

User Guide

| 37

Table 1.

Trace

Definitions

Trace

Module

Explanation

UV

 1–4

Single-Wavelength UV
and Multi-Wavelength
UV/Vis Detectors

UV — with Single-Wavelength UV module, a
reading of absorbance

MV — with Multi-Wavelength UV/Vis module, up
to four readings (

 1–4)

Conductivity

Single-Wavelength UV
and Multi-Wavelength
UV/Vis Detectors

The conductivity of the fluid, read by the
conductivity monitor (mS/cm)

%B Theoretical

System Pump

Programmed by the user in the method or in the
System Pump dialog box

Gradient, system pump: % of pump B

Gradient, blending valve: % of valve Q4

Buffer blending: % of valve Q4

pH Theoretical

pH

Theoretical pH, as programmed in the method or
System Pump dialog box. Available only with the
buffer blending valve in buffer blending mode

pH

pH

The measure of pH read from the pH probe,
available only when a pH valve is present

System
Pressure

System Pump

Measured at the mixer (psi or MPa)

PreCol Pressure

Column Switching Valve

Available when at least one column switching
valve is present

Tip:

When two or more column switching

valves are present, the traces appear in
different shades of green on the
chromatogram. The trace number
corresponds to the valve number in the fluidic
scheme. For example, PreCol Pressure 1 in
the legend and the chromatogram
corresponds to C1 in the fluidic scheme.

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