Bio-Rad ChromLab™ Software - Manual (Page 96)

4 | Performing a Manual Run
94 | NGC Chromatography Systems and ChromLab Software
Preparing the System
System preparation consists of priming the system to remove air from the pumps,
tubing, and valves followed by flushing the fluidics system to remove storage buffer,
cleaning solutions, or old buffer. Priming is performed in manual mode, while both
purging and cleaning tasks can be performed either manually or through
preprogrammed method phases. See Chapter 5, Method Editor for more
information about working with methods.
Priming and Purging the System
Perform the following procedures consecutively to prime and purge your NGC
systems.
Important: Whenever you add or remove a module or change or upgrade your
NGC system hardware configuration, you must replumb and reprime the
system.
To prime the system
1. Power on the NGC system and its connected computer.
2. To enter manual mode, do one of the following:
 On the computer, click Manual Run in the ChromLab Home window or
click the System Control tab and then click in the fluidic scheme.
 On the touch screen, click Enter Manual Mode if the application is not
already in manual mode.
3. (Optional) If your hardware configuration has changed, select a fluidic scheme
that matches your current system configuration.
a. Select Tools > Change Fluidic Scheme.
b. Select the appropriate fluidic scheme in the Fluidic Scheme Selector pane.
c. Click Select to choose the manual mode fluidic scheme.
4. Insert appropriate inlet lines into the buffer, storage solution, and/or cleaning
solutions to be primed and flushed through the system.