INFICON HAPSITE ER Chemical Identification System - Manual (Page 298)

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HAPSITE ER Operating Manual
9.5.2 How to Use Background Subtraction
WARNING
Be careful not to subtract a data/peaks that may be
needed for proper identification within the
chromatogram.
Background subtraction is a key component in "cleaning up" spectra before
attempting to search against a database such as NIST. In order to obtain
background spectra, there are two triangles ("cursors"), one with one blue line -
designated as Background 1 (B1), the other with two blue lines designated as
Background 2 (B2), located at the bottom left of the TIC window.
NOTE: All background subtractions are indicated in the Spectrum window by
the designation Scan Number - B1(range) - B2(range).
9.5.2.1 Steps Required to Perform a One Point Background Subtraction
Figure 9-10 Original Chromatogram
1 Using the Scan Cursor, select a peak of interest in the chromatogram. Refer to
Figure 9-10 .
2 Perform a manual NIST search by pressing the F7 key or by checking the
Search NIST/User box in the Control Panel. The search shows multiple
results. Refer to
Figure 9-10 .