Bio-Rad EXQuest Spot Cutter - Manual (Page 105)

PDQuest User Guide
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Invert Display
The Invert Display checkbox flips light bands on a dark background to dark bands on
a light background, and visa versa. Once, again, the actual data will not change—only
the image.
Highlight Saturated Pixels
When the Highlight Saturated Pixels checkbox is selected, areas of the image with
saturated signal intensity are highlighted in red. This setting does not apply to
Gaussian images.
3.13 Cropping Images
When matching spots between the gels in a MatchSet, the MatchSet images should be
all the same size and shape. If the original scans of these gels are different sizes, you
will need to crop them before creating the MatchSet.
Note: You can only crop Raw 2-D scans, not Filtered or Gaussian images.
The crop tools can also be used to reduce the file size of images. (To reduce
the file size of images by lowering the resolution, see Reduce File Size.)
Fig. 3-17. Crop commands.