Bio-Rad EXQuest Spot Cutter - Manual (Page 272)

Basic Excision Tool
8-7
Fig. 8-4. Spot cutter calibration step 2.
Step 2: The third dialog requires the calibration template grid pattern supplied with the
PDQuest system. Place the grid on top of the cutting mat, which should have
been previously installed on the cutter. Make sure the grid is flat and aligned with
the stage edges. Click Ready to proceed. You may also skip this step, return to the
previous dialog, cancel the action, or ask for help.
Step 3: The fourth dialog prompts you to place the coomassie stained PVDF membrane
on the mat. Click Yes or No.
At any point in the procedure you can move back to repeat a step.
Note: Calibration creates several parameter and image files in the Install.prm directory
in the Discovery Series directory on your hard drive. These files should not be
moved, deleted or altered, or calibration will be affected.