Bio-Rad EXQuest Spot Cutter - Manual (Page 90)

Viewing and Editing Images
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3.8 Density Tools
The tools under the View>View Density submenu and on the Density Tools toolbar
are designed for advanced users when analyzing gels. They provide a quick measure
of the signal intensity of the data in Raw 2-D scans or Filtered images. Note that these
commands will not work on Gaussian images.
Fig. 3-6. Density submenu and toolbar.
Density at Cursor
To display the signal intensity at a point on the image, select Density at
Cursor from the submenu or toolbar and click the image. The pop-up box
will list the intensity in image units (ODs or counts), and will also show the
average intensity for a 3 x 3 image pixel box centered on that point.
Density in Box
To display the total signal intensity inside a specified region on the image,
select Density in Box from the submenu or toolbar and drag a box around
the area you want to measure. The area of the box (in square millimeters),
the total image intensity (volume) inside the box (in image units—ODs or
counts), and the average intensity will be displayed in a pop-up box.