Bio-Rad EXQuest Spot Cutter - Manual (Page 317)

PDQuest User Guide
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Switching Gels
When all the spots on one gel have been cut, you will be prompted to place the next
gel on the spot cutter platform and align that gel image with the MatchSet image (see
above). Repeat the steps described above until the cut run is complete for each gel.
Ending the Cut Run
Click Pause to pause the cut run. Note that the cutter will pause only after the most
recent cut has been deposited in a well. Any remaining cut requests will remain
selected in the image. At this point, you can click Begin/Resume to complete the
remainder of the cuts, or abort the run.
When the cut run is finished, the cutting tip will return to home position. If you have
selected the Save Validation Image checkbox, the validation image will be acquired
and you will be prompted to save it. The validation image is a cutter camera image
showing the cut spots.
Recutting Spots
When all cuts are complete, visually inspect the cut holes for visible spots, or take a
UV image of the MTP and confirm the cuts as follows.
You can then select Cut checkboxes in the cut list to recut any missed spots or cut
spots that were skipped in the previous run. At any time, you can reopen an old cut list
in the Integrated tool and perform recuts or cut previously skipped spots.
As before, click Align to begin the cut run.