Bio-Rad EXQuest Spot Cutter User Manual

Page 252

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Analysis Sets and Annotations

7-25

should be selected. You can also create specialized categories using the URL arg and
Filename buttons (category types are described below).

When you click Done, the category is created and will appear selected in the Select
Category list.

To edit an existing category, select the category in the list and click the Edit button.
You can then change the category type and description. Note that the annotation entry
may be specific to a particular category type; changing the type may render the entry
meaningless.

To delete a category, select it, then click Delete. You will be warned before the
category and all its entries are deleted.

URL Categories

This type of category provides a link to an Internet protein database or other Web site;
then the annotation entry references the specific file or Web page for a particular
protein. You can create different URL categories to link to different databases,
including Swissprot, Unigene, and GeneCards.

To create a URL category, open the Add Category dialog box, enter a name for the
category, then select the URL arg option button and type a name for the database or
Web page in the Link Name field. This is the name that will appear in the Browse
Type the entire link address in the Link URL field including http://, any arguments,
with a %s where the individual spot annotations will go.. For example, Swissprot is
http://www.expasy.ch/cgi-bin/sprot-search-de?%s.

When you are finished creating the category, click Done. When you create an
annotation entry for a spot in this category, it should be the exact format necessary to
complete the URL link to the page in the database for the specific protein information
you are referencing.

To open a URL link, right-click on the spot and select Browse Annotations. The
HTML page for the spot will open, and the URL category name will be displayed as a
link. Click on this link to access the protein information in the database or Web site.

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