Bio-Rad EXQuest Spot Cutter - Manual (Page 345)

PDQuest User Guide
11-10
Specify the spots to include in your report: All Matched Spots or the spots in a
Specified Analysis Set dropdown list. If you click Analysis Set, click the drop down
arrow to display a list of available Analysis Sets. The name of the set you choose
appears in the field.
If you want to see spot quantitation for each individual member of the MatchSet,
select Individual Gel in the Quantitation section. If you want to see the average
quantitation for a Replicate Group, click the Replicate Groups.
You can sort your spots by SSP number, DSN, trend, ratio, molecular weight, or
isoelectric point by clicking on the appropriate Sort by button. To sort in ascending
order, check the box labeled Increasing.
Options
The following options can be included in the Quantity Table Report:
• If DSN numbers are assigned to the protein spots and you would like to see them
on the report, click the DSN button.
• MrpI data will be reported if you click the MrpI button.
• Check the box labeled Trend to view the trend of each spot.
• You can view the group count and the group critical value by checking Group
Count and GroupCV repectively.
• You can view the ratio of spots to a specific gel by clicking Ratio then selecting a
gel from the drop down list.
• You can print spot annotations by clicking the Annotation button and selecting
from the list of annotation categories. The report will include annotation
information for the spots that have annotation data in the selected category.
Other Options
Data - Choose whether to round values off the the closest integer or include noralized
values.
Missing Spots - You can choose to estimate missing spots, exclude them from the
report, or enter a minimum value to include in the report.
Saturated spots - You can choose to estimate saturated spots, exclude them from the
report, or enter a minimum value to include in the report.