Bio-Rad EXQuest Spot Cutter - Manual (Page 283)

PDQuest User Guide
8-18
Fig. 8-13. Specify the wells to re-cut.
In the pop-up box, enter the numbers of your recut requests (microplate number, well
number), separated by semicolons. Click OK to close the recut request dialog, then
click Begin/Resume to perform the recuts.
Re-cuts in Basic Excision Tool
The specification for the ProteomeWorks Spot Cutter spot pick-up is greater than
95% effective at picking up a spot that has been cut from a gel. There may be some
spots that have not been picked up and these can be re-cut. The efficiency of pick up
on the second cut is again >95%, so the spot is reliably picked up the second time.
The missed pick ups are detected by the user looking in the microtiter plate for empty
wells. However, with fluorescent gels, the clear gel spot (with no visible stain) is very
difficult to see in the microtiter plate wells. In order to assess the wells of the
microtiter plate, the re-cut message on the screen for the fluorescent light source will
differ from the white light message. Use the following procedure for re-cutting spots
in fluorescent mode:
When UV-Fluorescent imaging has been used on the spot cutter, at the end of the cut
run the software will prompt with, "Confirm cuts?"