Bio-Rad EXQuest Spot Cutter - Manual (Page 153)

PDQuest User Guide
5-2
A MatchSet can consist of one gel or many gels, depending on the type and size of the
experiment. The MatchSet is displayed in a single window, with subwindows for the
images of the member gels, and a subwindow for the MatchSet Master.
The Master is a synthetic image that contains the spot data from all the gels in the
MatchSet. All spot comparisons and analysis are coordinated through the Master.
Masters from different MatchSets can be grouped into higher level MatchSets for
further comparison (see section 5.8.b, Higher Level MatchSets).
Using a MatchSet, you can make quantitative and qualitative spot comparisons across
gels, calculate molecular weight/isoelectric point values, annotate spots, group them
into analysis sets, combine the data from multiple gels into replicate groups, and
select spots for excision and mass spec analysis.
You can also use a MatchSet to combine gels with different pH gradients into a larger
cybergel. See 5.6, Cybergels.
Fig. 5-2. Example of a MatchSet. The Master is in the upper left subwindow.
Note: While MatchSets can contain gels with different quantitation units, quantitative
spot comparisons can only be made between gels that have the same units (i.e.,