Bio-Rad EXQuest Spot Cutter - Manual (Page 481)

PDQuest User Guide
H-16
The imaging area will change depending on your zoom factor. For example, if you
have zoomed in on a area that is 4.5
x 3.5 cm, then you would enter 4.5 for the width
(3.5 for the height would be calculated automatically).
Note: Your imaging area settings must be correct if you want to do actual-size printing.
They must also be correct if you want to compare the quantities of objects (e.g.,
using the Volume Tools) in different images.
The imaging area dimensions also determine the size of the pixels in your image (i.e.,
resolution). A smaller imaging area will result in a higher resolution.
H.6.e Auto-scale Transform
Auto-scale Transform after Acquisition allows the user the option of having the image
automatically perform the Auto-Scale transform function upon completion of image
acquisition. This eliminates the need to transform an image or re-scan an image when
the acquisition time was too short or the iris not opened enough.
To enable the auto-scale transform function, check the box labeled, Auto-Scale
Transform after Acquisition in the Options dialog.
H.7 Other Features
Fig.H-12. Other Fluor-S MAX acquisition window features.
Highlight Saturated Pixels
When this box is checked, any saturated pixels in the image will appear highlighted in
red in the scan window and in the pop-up image window. To view/hide saturated
pixels in the pop-up image window, use the Image > Transform command.