Bio-Rad EXQuest Spot Cutter - Manual (Page 183)

PDQuest User Guide
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Graph Partial Matches displays histograms of each partially matched spot in the
Master. Select the command from the submenu and click in the Master. A missing bar
in the histogram indicates that the spot is unmatched in a MatchSet gel.
Fig. 5-18. Graph Partial Matches; each bar represents the spot’s quantity in a gel.
Note: Histograms can only be displayed for one region of an image at a time. Use the
Reports > Graph Advance (Alt+.) and Reports >Graph Retreat (Alt+,) commands
to shift between regions. Or you can navigate to the View menu and select the
graph toolbar.
Erratic Spots
Erratic spots are matched spots whose quantities vary significantly between member
gels. To mark these, select Mark Erratic Responses from the Match > Edit Matches
submenu and click in the Master. Enter a fold change factor in the pop-up box. (For
example, a change factor of 3 will highlight spots whose quantity varies by a factor of
3 or more among member images.) When you click Apply the selected spots will
appear circled in the Master.
Graph Erratic Responses displays histogram graphs of each erratic spot in the Master.
Select the command from the submenu, click in the Master, enter a fold change factor
in the pop-up box, and click Apply to display the graphs.
Missing bars indicate
matched in some
that the spots are not
members.