Bio-Rad EXQuest Spot Cutter - Manual (Page 136)

Detecting and Editing Spots
4-15
Note: Spots without crosshairs in the Filtered and Raw 2-D Scan images have not been
detected and are not included in the Gaussian image.
Fig. 4-9. Examples of crosshairs and ellipses in a scanset.
Show Ellipses (Alt+F5, Spots menu) shows the shape of each spot generated by
Gaussian fitting. Use the tool or the key command in the subwindow to toggle the
display. Show All Ellipses (Ctrl+F5, Spots menu) shows the spot ellipses in all the
subwindows.
Hide Overlays (F4, main toolbar) will hide the crosshairs and ellipses on the image.
Use the tool or the key command in the subwindow to hide the overlays.
4.5 Adding and Removing Spots
If spot detection has missed some spots or identified too many spots in a cluster, you
can add and remove spots in the Gaussian image.