Bio-Rad EXQuest Spot Cutter - Manual (Page 314)

Integrated Excision Tool
9-25
Cut Area displays a green box on the cutter camera image; this shows the boundary
of the cutting area. The cutting head cannot cut spots outside this area.
Multiple Cuts displays the multiple cuts of large spots containing more than one cut.
Data box displays the green data area box. Uncheck this box if the data area box is
obstructing the view of a spot on the periphery.
The Compute cut overlaps function automatically calculates
overlapping spot areas. You can manually adjust overlapping
spots by placing your cursor on the center of an overlapping
spot and moving it to the left or right.
Display Cut Overlaps marks overlapping spots with ellipses in the MatchSet image,
and the percent overlap will be displayed next to the overlapped cluster.
Beginning a Cut Run
When all the cut spots have been aligned and confirmed in the Align Images dialog
box, click the Begin/Resume Cutting button. If you have not manually and visually
confirmed the spot alignment with Jump and Confirm, you are prompted to either go
back and do so, or proceed.
Fig. 9-14. Not all spots confirmed warning
If you proceed without confirmation it may result in reduced positioning accuracy.
You will be prompted to place a microplate in the plate holder. When you click OK in
the prompt box, the cut run will begin. The Integrated Excision tool dialog greys out
while it is working.