Bio-Rad EXQuest Spot Cutter - Manual (Page 130)

Detecting and Editing Spots
4-9
Click OK. The parameter set will now be available for processing all similarly
detected gel scans.
To load previously saved parameters, click the Load button and select from the list of
parameter sets. When the parameters are loaded, you can proceed to Find Spot
Centers on the loaded image. (Each parameter set is saved as an “.adp” file in the
Discovery Series <user name>.prm directory. To delete a parameter set, delete its .adp
file.)
4.1.e Processing Gels
Click the Process All Gels button to close the Spot Detection Wizard and open the
Detect Spots dialog box.
If you have not saved the current detection parameters, you will be prompted to do so
before you exit the Wizard.
The Quit button will close the Wizard without opening the Auto Detection and
Matching window. If you have not found spot centers, any unsaved parameters will be
lost.
4.2 Detecting Spots
After you have created and saved a set of detection parameters using the Spot
Detection Wizard, you are ready to detect spots on your images. In general, you can
use the same parameters to detect spots on all gels that use the same stain and have
been run and scanned in the same manner.
Click Process All Gels in the Wizard to open the Automated Detection and Matching
Detect Spots dialog box. You can also open the dialog directly by selecting
Automated Detection and Matching tool form the Spots menu. All currently loaded
gel scans will be listed in the panel.