Bio-Rad EXQuest Spot Cutter - Manual (Page 297)

PDQuest User Guide
9-8
The spots selected to be cut are highlighted in the table in blue. The table will initially
show one cut request per spot—that is, one highlighted blue table cell per row.
If you click the Standard Spot Number (SSP) at the beginning of each row, that spot
will appear highlighted in the actual MatchSet. See Figure 9-2 “Excision Gel
Selection.” for a pictoral representation. Study the size and quality of each spot in the
gels in the MatchSet when selecting spots to cut.
You can manually add or remove cut requests by clicking on the table cells. Click on
a blue cell to delete a cut request. Click on a white cell to make a cut request. To cut
the same spot in different gels, click on multiple white cells in the same row; they will
be highlighted in blue. You can add as many cut requests in as many gels in the
MatchSet as you want.
At the bottom of the table, the total number of cut requests per gel is shown.
9.1.d Performing the Cuts
When you have made your selections, click the Next button to close the Excision Gel
Selection tool and open the Integrated Excision Tool.
9.2 Integrated Excision Tool
The Integrated Excision Tool is used to cut a set of spots from the gels in a MatchSet
using Bio-Rad’s ProteomeWorks Spot Cutter system. It provides all the features of the
Basic Excision Tool, plus the ability to cut spots from PDQuest MatchSets.
Before using this tool, you must have selected the spots and the gels to cut using the
Excision Gel Selection tool, as described above. The MatchSet containing the gels
must be open and active. Also, make sure the spot cutter is connected with the
computer, is switched on and has warmed up, and make sure the camera is connected
and has been turned on.
Note: See the spot cutter hardware manual for instructions on installing the platform,
camera, and associated hardware and software.
Select Integrated Excision Tool from the Identification menu. The software will
connect with the spot cutter platform and camera, and the Integrated Excision Tool
dialog box will open.