Bio-Rad EXQuest Spot Cutter - Manual (Page 277)

PDQuest User Guide
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If this is the first cut added, the Enter Plate Information dialog box will open first.
when all the pertinent information is added, the cut will be added to the image.
Fig. 8-8. Enter Plate Information dialog.
Enter a name for the microplate and identify the first well you want to load. If you
specify a starting well other than the default of A1, the skipped wells will be noted in
the dialog box. Click Done when you have completed your selections.
A green circle will appear on the image, indicating the spot to be cut. Note that the
circle is the size of the actual cutting tip (1.0 mm or 1.5 mm, as specified in the Spot
Cutter Settings dialog box).
Continue clicking on spots in the image with Add Cut selected to make additional
cuts. The number of cuts will appear in the Cuts Selected field.
If you specify more cuts than there are wells in the microplate, you will be prompted
to name a new plate and starting well for subsequent cuts.
Moving a Cut Circle
To move a cut circle, make sure that Add Cut is selected, then position your cursor
inside the cut circle. The appearance of the cursor will change to a multidirectional
arrow. Drag the circle to a new location and release the mouse button.