General operation – Bio-Rad EXQuest Spot Cutter User Manual

Page 50

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General Operation

2-19

Fig. 2-12. Reduce File Size dialog box, before and after pixel size increase.

Note:

Asymmetric pixel reduction (i.e., making pixels smaller in one dimension than
the other) is not recommended for 2-D gels, since you want to resolve spots in
both dimensions.

A pop-up box prompts you to confirm the resolution reduction. If you choose to
reduce in place, the descriptions of objects in the scan will be lost. Only the scan
image is retained. Click Reduce and Copy to make a copy the original file and reduce
the copy’s size, or click Reduce to reduce the file size of the displayed image.

Reducing the file size is an irreversible process. For that reason, we suggest you make
a copy of the image and reduce its file size. That way, if you lose too much resolution,
you can simply delete the copy and try again. Once you are happy with the reduced
image, don’t forget to delete the original. The goal is to save space!

Pixel size increase confirmation

Pixel Count and Memory Size reduced

Before:

After:

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