Bio-Rad EXQuest Spot Cutter User Manual

Page 301

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PDQuest User Guide

9-12

Fig. 9-5. Spot cutter settings dialog box.

In the dialog box, select the communications port to the spot cutter by clicking on
COM 1 or COM 2.

Under Miscellaneous, specify the microtiter plate size you are using by clicking on 96
wells or 384 wells.

Specify the diameter of the cutting tip by selecting 1.0 mm or 1.5 mm. Note that the
cut request circles that you make on the image reflect the actual size of the cutting tip,
and will change based on the tip size selection.

Specify the order in which you want to the load the wells on the microplate by
selecting A1, A2, A3, ... or A1, B1, C1, ...

Crop Left Margin repositions the image in the Basic Excision Tool window to the left.
Enter a value (in screen pixels) in the field to reposition the image. Note that you must
click Done to close the settings dialog and then acquire a new image for your changes
to take effect.

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