Bio-Rad EXQuest Spot Cutter - Manual (Page 536)

Appendix L. Techniques
L-11
Fig. L-5. Diagrammatic representation of merging.
Cropping and Aligning Exposures of the Same Gel for Merging
Before you can merge multiple exposures, you must crop and align them so that they
are exactly the same size and position. To do this, you will use the Advanced Crop
commands on the Image menu. See section 3.13.a for instructions.