Bio-Rad EXQuest Spot Cutter - Manual (Page 172)

Matchsets
5-21
Set the matching precision slider towards strict or liberal, depending on the degree of
precision you prefer. The most rigid setting you can choose is 0 and the most flexible
setting you can choose is 100. The number 50 is the average between the two
extremes. The ratio to which you adjust the slider is displayed in the white box to the
right of the slider.
Select matching settings from the default settings listed in the dropdown menu. Select
the gel members you want to match from the list in the Matching Parameters pop-up
box.
Select the gel members you want to match from the Select Members pop-up box.
As automated matching proceeds the numbers of Matches, Extensions, New Spots,
and Sum total display in the appropriate boxes.
To auto-match all the gels in the MatchSet, select Classic Match All Gels from the
Match menu or toolbar. To auto-match a single gel with the Master, select Classic
Match Gel from the Match menu and click in the gel subwindow. Or click the Match
All button at the bottom of the Automated Matching dialog. Click the Done button
when you have completed your matches.
Extended Matching
Use Extended Matching to choose the method you want to use to extend primary
matches. Your options in the Extension method dropdown list are Disabled,
Restricted, or Classic. The color of the match symbol for extended matching is blue.
5.5.a Show Symbols
Match > Show Symbols highlights all the different types of matches and
unmatched spots using different labels. Select the tool from the Match
menu or toolbar and click in the Master or member gel subwindow, or use
the F7 key command in the appropriate subwindow.
• Auto-matched spots are labeled with green letters.
• Manually matched spots are labeled with purple boxes.
• Unmatched spots are circled in red. These are also displayed using the Mark
Unmatched Spots command (Shift+F8) on the Match >Edit Matches submenu.
• Landmarks are labeled with green triangles. These are also displayed using the