Bio-Rad EXQuest Spot Cutter - Manual (Page 221)

PDQuest User Guide
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Entering MrpI Values for Known Spots
To determine MrpI values for all the spots in the MatchSet, you first need to enter the
values for a few known spots.
Select Enter MrpI Data from the Analysis > MrpI Standards submenu or toolbar, then
click on a spot in the MatchSet Master whose molecular weight and/or isoelectric
point values are known. A dialog box prompts you to enter the values for that spot.
Fig. 6-22. Pop-up box in which MrpI data are entered.
Type the molecular weight first, followed by a space, followed by the isoelectric
point. Click OK when you are done.
If you make a mistake entering the data, you can select Enter MrpI Data again and
click the spot. The values you entered will appear in the dialog box and you can make
the necessary corrections.
If you want to remove the MrpI data for a spot, select Remove MrpI Data and click
the spot.
Displaying MrpI Data
To see the proteins for which you have entered MrpI data, you can use either the Mark
MrpI Spots or Display MrpI Data option. The first option highlights the spots for
which you entered data. The second option displays MrpI values in red next to the
spots.