Bio-Rad EXQuest Spot Cutter - Manual (Page 340)

Graphs and Reports
11-5
11.2.a Data Displayed in the Histogram
Each bar in a histogram for a spot represents the spot’s quantity in a member of a
MatchSet. If replicate group quantitation is selected, each bar represents the spot’s
quantity in a replicate group.
The bars from left to right represent the gels in the order in which they were loaded.
As long as the spot is matched to the Master, you can display a histogram for it.
Fig. 11-6. Sample histogram.
The Standard Spot (SSP) number is displayed beneath the histogram.
The number at the upper right of the histogram is the quantitation of the maximum bar
in the graph. The other bars are drawn proportional to the highest bar.
Beneath the maximum quantitation, the normalization units are displayed.
Normalization is a process by which quantitative data from different gels are adjusted
so that you can compare different samples to one another (for more information on
normalization, see section 6.6).
If a spot is saturated, the bar representing it on the histogram will be shaded red
instead of black.
If replicate groups are defined for the MatchSet (see section 6.4), you can look at the
quantitation of individual spots or the average data of duplicate gels. You can specify
which way you want to review spot quantitation by selecting choosing a configuration
in the Configure Graphs dialog. See Section 11.1 for further information.
If you specify All Replicate Groups, the bars on the histograms will display not only
the maximum spot quantitation for each replicate group, but also the average spot
quantitation and the standard deviation. Each bar represents one replicate group. The
order of the bars corresponds to the order in which the replicate groups were made.
See Section 11.1, “Configure Graphs” for more information.
Maximum Quantity
Normalization Units