Bio-Rad EXQuest Spot Cutter - Manual (Page 167)

PDQuest User Guide
5-16
Fig. 5-11. Landmarking a spot.
If the program is unsure about positioning the landmark, the area in which the spot
was expected will be circled. Locate the appropriate spot and click on it.
As each landmark is placed, the cursor will jump to the next gel. Landmark the spot in
all members of the MatchSet, except when creating a Master out of gels with different
pH gradients; see 5.6, Cybergels.
If you accidentally landmark the wrong spot in a gel, simply go back and click the
correct spot. The landmark will move.
To remove a landmark from all the MatchSet gels, select Unlandmark from the Match
menu or toolbar and click the incorrect landmark in the Master. To remove a landmark
from a single gel, click in that gel.
To highlight all the landmarks in a MatchSet, use the Mark Landmarks command (F8,
Match menu, Match toolbar) in the Master image.
As you add landmarks, study the auto-matching in all regions of the gels. Add more
landmarks to regions where auto-matching has missed spots.