Bio-Rad EXQuest Spot Cutter - Manual (Page 307)

PDQuest User Guide
9-18
Select the wash stations to use after each cut by clicking on one or more checkboxes
(Station 1, Station 2, etc.).
Under Misc. Options, select Multiple Cuts/Spot to specify multiple cuts of larger
spots. The number of cuts/spot is selected in the Excision Gel Selection window. Note
that the software will automatically calculate the size of each spot based on its
Gaussian profile in the MatchSet gel when determining whether to make multiple
cuts.
Save Validation Image will create and save an image of the gel on the spot cutter
platform when the cut run is complete. You will be prompted to name the validation
image.
Step 3. Select the Microtiter Plate
Click the Select microtiter plate button to select a plate name, size, and starting well
for the current cut run.
In the dialog box, a default plate name will appear in the Plate Name field. Note that
the loading order of the plates are named sequentially (Plate 001, Plate 002, etc.) by
default. However, you can enter any name you want in the field.
Click the Specify Starting Well button and, in the pop-up box, specify the first well
you want to load in the current cut run. If you specify a starting well other than the
default of A1, the skipped wells will be noted in the box. Click Done to make your
selection.
Note: Specifying a different starting well is essential if you want to load spots from
different MatchSets or different analysis sets on the same microtiter plate. You
would load your spots from one MatchSet/analysis set on the plate, then select a
different MatchSet/analysis set, skip the previously loaded wells.
The Current Run Serial field displays the serial number of the cut run. The cut run
serial number increments whenever you perform a previous cut run on a new
MatchSet gel or gels. For example, if you first cut spots from one gel in a MatchSet
and do a cut run, then cut the same spots from a different gel in the same MatchSet
and do a run, the serial number for the second run would be 2.
When you have made your selections, click OK to complete the microplate selection.