Bio-Rad EXQuest Spot Cutter - Manual (Page 71)

PDQuest User Guide
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If you want to apply a uniform background to Gaussian images so they more closely
resemble the original gels, enter a background optical density (O.D.) value in the text
box next to the Gel Spot Bkgd prompt.
In Windows, Jump Cursor on Alert will automatically jump the cursor to the OK
button when an alert box pops up. Deselect the checkbox to turn off this feature.
By default, a subwindow is selected when you move your cursor over it. If you would
rather click a subwindow to select it, select Click to Focus Subwindow.
Note: Click to Focus Subwindow will change the behavior of mouse-assignable tools.
Some commands that were previously mouse-assignable will be performed
immediately on the active subwindow; other commands will require you to click
once in a subwindow to select it, then click or drag again to perform the action.
Auto ‘All Same’ duplicates the zoom and positioning commands made in one
subwindow in all displayed subwindows. For example, with Auto ‘All Same’
selected, Zoom In on one subwindow; the other open subwindows will also zoom in.
The Save subwindow configuration saves subwindows in the last order displayed.
If Bold Match Symbols is selected, landmarks, manual matches, added spots, etc. will
appear larger and bolder on the screen.
If Filled Spot Ellipses is checked, marked spots (e.g., spots marked using the
Saturated Spots command) will be displayed as filled ellipses. If unchecked, they will
be displayed as unfilled ellipses.
Solid Label Background will automatically display spot labels in a filled-background
box.
If Small Window Labels is checked, any text in the windows/subwindows will be
displayed in a smaller font.
Show Filtered Images by Default will automatically display the Filtered images when
you first open a MatchSet (provided the MatchSet includes Filtered images).
In Gaussian images, specify whether you want to see only single spots or multiple
spots by clicking on the appropriate button under Display combined spots. Note that
valid spots are spots that have not been cancelled. If you elect to display combined
spots, they will appear in the Gaussian image as they do in the Filtered image,
although the quantitation will be combined.