Bio-Rad EXQuest Spot Cutter - Manual (Page 179)

PDQuest User Guide
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threshold value directly into the box to the left of the slider. As you adjust the
threshold, spots that no longer meet that threshold will be deselected.
Set the filter range by selecting the Max option button (up to the maximum peak value
in the gel) or the Avg button (up to the average peak value).
The third option in the Auto-Add spot to Master dialog is the Filter By Quality
setting. This setting is similar to Filter by Peak Value, except that it filters by spot
quality.
The number of Spots selected appears below the Selection Criteria. Inside master
shows the number of spots that fall within the area of the current Master image, while
outside master shows the number of spots that fall outside that area.
Check Mark Spots to be added to select the exact position in the master gel you want
to add the spot.
Check Mark add spot location in Master to mark the spot in a member you want to
add to the Master.
Check Auto-configure MatchSet subwindows on launch to automatically configure
the subwindows in the MatchSet to imitate the Master.
You can select the style, color, and symbol for how you want your spots to display by
clicking the Format button.
When you are ready to add spots from the gel, click the Proceed button. The selected
spots will be added and the gel will be rematched with the Master. If some spots could
not be added because they were too close to existing spots you will be prompted to
rematch the gels by a pop-up box. You can add these spots manually.
To remove all spots added from the selected gel, click Undo Add. You will be warned
before the action is completed.
Manually Adding Spots
To manually add spots from a member gel to the Master, first display the unmatched
spots in the gel using Show Symbols (F7) or Mark Unmatched Spots (Shift+F8). Use
the Zoom Box tool to magnify a spot.