Bio-Rad EXQuest Spot Cutter - Manual (Page 529)

PDQuest User Guide
L-4
For example, you might begin with a 50 µg/ml stock solution of BSA and make
segments of the calstrip starting at 50 µg and progressing down to 5.0 ng in constant
ratio steps.
In order to enter numerical values in the Calstrip Record that will produce
interpretable spot quantitation values, convert all the numbers to ng (i.e., multiply all
µg values by 10
3
).
For the example mentioned above, the values would range from 50,000 ng to 5.0 ng.
Saving/loading Calstrip Records
When you have completed the information in the dialog box, click on the Save button
at the bottom of the box.
To access the information in an existing Calstrip Record, click on the Load button. A
list of saved Calstrip Records will be displayed. Select the one whose data you wish
to see by clicking on it. The data associated with that record will be displayed on the
form.
To delete a saved Calstrip Record, click on the Delete button.
Click on the Cancel button to close the Calstrip Record Editor form.
L.1.b Scan Information
After the calibration data has been entered, you will also need to enter some
information about the gel that goes with it.
Start by loading the 2-D and calstrip scans. Display the calstrip scans by using the
Configure Calstrip Windows function (Edit > Calibrate Gels > Configure Calstrip
Windows).
Click on Gel Record in the Calibrate Gels submenu and click on the calstrip scan. The
Gel Record dialog box will open, in which you can enter information about the
calstrip.