Bio-Rad EXQuest Spot Cutter - Manual (Page 141)

PDQuest User Guide
4-20
Fig. 4-13. Creating a spot contour.
To edit the boundary, position your cursor on the border with the contour tool. Your
cursor will change to a pencil. Drag across the line; a new yellow line will appear. The
boundary will look blue when not selected, yellow when it its being edited and green
when it is selected.
Spot Freehand Tool
To use this tool, first magnify the spot you want to quantify (you must be
able to see the individual pixels in the image). Then select the Spot
Freehand Tool from the toolbar or submenu, and click and drag around the
edge of the spot to enclose it in a boundary. When your line crosses itself, a
freehand boundary is created.
If you make a mistake while drawing, simply backtrack with the mouse. The line you
draw should completely surround the spot you want to quantify.
1. Select the contour tool.
2. Drag a contour around
the spot in the Filtered or Raw
2-D image.
3. The contour is created
and the spot is added to the
Gaussian image.