Bio-Rad EXQuest Spot Cutter - Manual (Page 184)

Matchsets
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Fig. 5-19. Graph Erratic Responses; each bar represents the spot’s quantity in a gel.
Note: Histograms can only be displayed for one region of an image at a time. Use the
Graph Advance (Alt+.) and Graph Retreat (Alt+,) commands to shift between
regions.
5.8.a Colony Matching
If you have a MatchSet containing colony plate images, you can match colonies
quickly and easily using the Colony (two-point) Match command on the Match > Edit
Matches submenu.
Unlike the regular matching function, you only need to define two landmarks on your
member images to use this command.
Note: Also unlike the regular matching function, this command automatically corrects
for any rotational errors in the imaged plates (common in colony plates). You do
not need to perform additional image rotation.
After you have created a MatchSet and defined two landmarks in the member images
(see above), select Colony (two-point) Match from the submenu and click on a
member image to automatically match the colonies. The other matching commands
also work in colony plates.
Removing Matches
To remove all automatic matches from a member image, select Remove Auto
Matches from the Match > Edit Matches submenu and click on the member image.
Histogram bars reveal
a three-fold variation in
spot quantity between
gels.