Bio-Rad EXQuest Spot Cutter - Manual (Page 173)

PDQuest User Guide
5-22
Mark Landmarks command (F8) on the Match menu and toolbar.
• Spots added to the Master are labeled with blue boxes. These are also displayed
using the Mark Added Spots command (Alt+F8) on the Match menu and toolbar.
To highlight individual matched spots, select Show Match from the Match
menu or toolbar and click on a spot in the Master, or use the F9 key command
on the spot. The spot will appear highlighted with a yellow box in all the gels
in which it has been matched. In gels where it is unmatched, a circle will
appear where the spot would be located.
5.5.b Show Vector Offsets
Vector offsets are lines showing the distance and direction between the spot centers in
the member gels and the corresponding spot centers in the Master. They provide a
visual tool for judging gel alignment and distortion and the accuracy of matching.
Select Show Offsets from the Match menu or toolbar and click in a
subwindow to display the offset lines, or use the Shift+F7 key command in
the subwindow.
Look for the following conditions that indicate possible matching problems:
• Offset lines that cross each other (this is a key problem).
• Grossly nonparallel lines in local regions.
• Lines of unequal length in local regions.
• Areas of the gel with few offsets; this indicates unmatched regions.