Bio-Rad EXQuest Spot Cutter - Manual (Page 132)

Detecting and Editing Spots
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As indicated on the dialog, you can set the order of the members by dragging them in
the list. This order will be used when displaying the images as well as plotting bar
graphs.
Step 2 - Detect Spots on Gels
This will autmotically process all the selected images. If you have processed similar
images in the past, you may select the same parameter set with the drop-down list
button.
If you can not use an existing parameter set, press the Edit... button to launch the Spot
Detection Wizard and create a new parameter set.
Step 3 - Create MatchSet
Follow the same procedure as you did when you created a New MatchSet to enter the
Name, Description, Comments, and Member Sharing.
To specify the directory in which the MatchSet will be stored, click Change.
Note: Note:If you want to detect spots without creating a MatchSet, check the "Disable
this step" checkbox at the bottom of Step 3.
Step 4 - Match Gels
This is similar to Step 2 in that you may select existing parameters for matching or
you may create new ones with the Edit... button. However, unlike Spot Detection
parameters, the matching parameters work over a wide range of images and you will
probably obtain satisfactory results with the default parameters.
Note: Note:If you want to detect spots without creating a MatchSet, check the "Disable
this step" checkbox at the bottom of Step 4.
Spot Detection Report
To review the log file created during spot detection, select Spot Detection Report
from the Spots menu. This report documents when spot detection began, which gels
were detected (or failed to detect), which steps were performed, and when spot
detection was completed.