B member sharing – Bio-Rad EXQuest Spot Cutter User Manual

Page 157

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PDQuest User Guide

5-6

Raw 2-D and Filtered images are in included in the MatchSet by default. Including
the Filtered images allows you to compare the Gaussian spots with the original spots
while matching, and edit the results of spot detection. (Invariably, during matching
you will notice missed or misdetected spots.)

To view the filtered image click in the Gaussian or Filtered image subwindow and
toggle from one version to another. Use the Interchange Image (F11) command or
Interchange All Images (Shift F11). Additionally, you can place your cursor in the
subwindow you want to toggle and right-click to display the Quick Reference Guide.

Fig. 5-5. Interchange Images

5.1.b Member Sharing

Under Member sharing, decide whether you want to make copies of the member files
or use the originals.

If you choose “Refer to Originals” you will save disk space by not making copies of
the member image files. Note that you may include the same member files in more
than one MatchSet. If you do, edits such as adding or removing spots will affect all
MatchSets that include these files.

On the other hand, if you choose “Make Copies” the files will be copied to this
MatchSet’s data directory. This enhances the possibility that edits made on the

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