Bio-Rad EXQuest Spot Cutter - Manual (Page 518)

Appendix K. VersaDoc
K-11
To enable this feature, select the Flat Fielding checkbox.
Note: For applications using the UV or white light transillumination, you should use
the appropriate reference plate to ensure a uniform intensity in the image. This
will compensate for normal variations in image pixel intensity that occur with a
transilluminating light source.
UV Illumination Flat Fielding: When you first select the Flat fielding checkbox, and
then acquire an image using the UV transilluminator, you will be prompted to remove
your sample and place the fluorescent reference plate on the VersaDoc sample stage
(see the VersaDoc User Manual). A reference image of the plate will be acquired and
saved on your computer hard drive. The reference image will be applied to the sample
image to generate a Flat Field corrected image.
White Illumination Flat Fielding: When you first select the checkbox, and then
acquire an image using the white light conversion screen, you will be prompted to
remove your sample and collect an exposure of the conversion screen. A reference
image of the screen will be acquired and saved on your computer hard drive. The
reference image will be applied to the sample image to generate a Flat Field corrected
image.
For subsequent UV or white light trans exposures, you will be prompted to either use
the appropriate saved Flat Fielding image or acquire a new one. Any changes in light
source, filter, or lens setting will require the acquisition of a new Flat Fielding
reference image.
Acquire
Click on the Acquire button to capture a single image for each enabled channel. An
exposure will be taken for each enabled channel based on the time selected for that
channel in Step III.
The progress of each exposure will be displayed in the Exposure Status bar at the
bottom of the acquisition window.