Bio-Rad EXQuest Spot Cutter User Manual

Page 316

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Integrated Excision Tool

9-27

Fig. 9-16. Progress completed on six cuts in the cut run.

Confirming Cuts

The Confirm button helps you check the cut progress of a
fluorescent stained gel. With visible stained gels it is easier to look
in the spot cutter to confirm that the cutting tip is cutting the spot

accurately. Since the spots are not visible without UV illumination in a fluorescent
gel, the Confirm function provides an on-screen method for monitoring the cut run.

After one or more cuts have been made, click Pause to pause the cut run. Note that the
cutter will pause only after the most recent cut has been deposited in a well.

Confirm the pause, then click the Confirm button under the main acquisition window.

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