Bio-Rad EXQuest Spot Cutter - Manual (Page 226)

Analysis Tools
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3-4=C 8.0-8.5=L
4-5=D 8.5-9.0=M
5.0-5.5=E 9-10=N
5.5-6.0=F 10-11=P
6.0-6.5=G 11-12=Q
6.5-7.0=H 12-14=R
DSNs have the format ABnn. “A” is the molecular weight grid letter, “B” is the
isoelectric point grid letter, and “nn” is the number of the spot within that section of
the grid.
Assigning DSNs
After you have entered MrpI values for known spots, you can create a DSN grid using
this data by selecting Assign DSN Grid from the Analyze > Spot Numbers submenu.
This automatically assigns a DSN number to each spot in the Master.
Fig. 6-25. DSN grid, counts, and numbers.
To display the grid (this does not occur automatically when the grid is created), click
in the Master and select Plot DSN Grid. A grid of vertical and horizontal axes will
appear, with the letter assignments of each axis displayed in the window margins.
To see the spot assignments, select Display DSNs. To read the numbers, you will
probably need to zoom in on a smaller region of image.
Transfer DSNs is used to copy the DSN numbers from one higher level MatchSet to
another See section 5.8.b, Higher Level MatchSets.
Plot DSN Grid Plot DSN Grid Counts Display DSNs